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Reference: GF1014F / KL1014F

Bismuth Sulfite Agar

Selective plating agar for the separation and culture of Salmonella, with bismuth, sulfite and brilliant green in the formula.

Key specifications

Product code (dehydrated powder)
GF1014F
Product code (granulated)
KL1014F
Form
Dehydrated powder (GF) and granulated (KL) versions
Quantity per litre of distilled or deionized water
50.3 g
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Bismuth Sulfite Agar is a selective solid medium for separating and culturing Salmonella. Its formula combines peptone, beef extract and glucose with ammonium bismuth citrate, sodium sulfite, ferrous sulfate and 0.025 g/L brilliant green, set with 20 g/L agar.

Suspend 50.3 g in 1 L of distilled or deionized water and boil with frequent agitation until dissolved. Cool to 45-50 °C, shake well and pour into sterile plates. The medium must not be overheated or autoclaved. The final pH is 7.5 ± 0.2.

Dehydrated powder (GF1014F) and granulated (KL1014F) versions are listed with the same composition. The product is not for clinical use.

Specifications

Technical specifications

18 parameters

Identification

Product code (dehydrated powder)
GF1014F
Product code (granulated)
KL1014F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of distilled or deionized water
50.3 g
Dissolution
Heat with frequent agitation and boil to dissolve completely
Pouring
Cool to 45-50 °C, shake well and pour into sterile plates; do not overheat or autoclave
Final pH (25 °C)
7.5 ± 0.2

Storage

Storage
Store in a dry place away from light; tighten the cap immediately after use
Shelf life (sealed)
3 years

Composition

Peptone
10 g/L
Beef Extract
5 g/L
Ferrous Sulfate
0.3 g/L
Ammonium Bismuth Citrate
2 g/L
Sodium Sulfite
6 g/L
Disodium Hydrogen Phosphate
4 g/L
Glucose
5 g/L
Brilliant Green
0.025 g/L
Agar
20 g/L
Features

Key features

  • 01

    Salmonella separation

    Used for the selective separation and culture of Salmonella.

  • 02

    Bismuth-sulfite system

    Contains ammonium bismuth citrate, sodium sulfite, ferrous sulfate and brilliant green.

  • 03

    No autoclaving

    Heat to boiling to dissolve only; do not overheat or autoclave.

  • 04

    Plate-ready

    Cool to 45-50 °C and pour into sterile plates.

  • 05

    Shelf life

    Sealed product keeps for 3 years, dry and away from light.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.