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Reference: GF1013F / KL1013F

Brain Heart Infusion Broth (BHI Broth)

Infusion-based broth for enriching Staphylococcus aureus and culturing fastidious or nutritionally demanding bacteria; the dehydrated powder carries an ISO 6888 reference.

Key specifications

Product code (dehydrated powder)
GF1013F
Product code (granulated)
KL1013F
Form
Dehydrated powder and granulated forms
Standard reference (ACC, dehydrated powder)
ISO 6888
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Brain Heart Infusion (BHI) Broth is a nutrient-rich liquid medium prepared at 37.0 g per litre with a final pH of 7.4±0.2. The dehydrated powder (GF1013F) is stated for the enrichment of Staphylococcus aureus and the culture of fastidious organisms, carries an ISO 6888 reference and is made from calf brain and beef heart infusions with an enzymatic digest of animal tissues.

The granulated form (KL1013F) is stated for the pure culture of Staphylococcus aureus and of nutritionally demanding bacteria, and uses a tryptone and beef heart powder formulation. Both are autoclaved at 121 °C for 15 minutes. Shelf life is 5 years for the dehydrated powder (stored at 2-25 °C) and 3 years for the granulated form.

Specifications

Technical specifications

21 parameters

Product

Product code (dehydrated powder)
GF1013F
Product code (granulated)
KL1013F
Form
Dehydrated powder and granulated forms
Standard reference (ACC, dehydrated powder)
ISO 6888

Preparation

Preparation (both codes)
Suspend 37.0 g in 1 L of distilled water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 15 min.
Final pH (25 °C, both codes)
7.4±0.2

Storage

Storage (dehydrated powder)
Store in a dark and dry place at 2-25 °C; tighten the bottle cap immediately after use.
Storage (granulated)
Store in a dry place away from light; tighten the cap immediately after use.
Shelf life, sealed (dehydrated powder)
5 years
Shelf life, sealed (granulated)
3 years

Composition (dehydrated powder)

Enzymatic digest of animal tissues
10.0 g/L
Dehydrated calf brain infusion
12.5 g/L
Dehydrated beef heart infusion
5.0 g/L
Glucose
2.0 g/L
Sodium chloride
5.0 g/L
Disodium hydrogenphosphate, anhydrous
2.5 g/L

Composition (granulated)

Tryptone
10 g/L
Beef heart powder
17.5 g/L
Sodium chloride
5 g/L
Glucose
2 g/L
Disodium hydrogen phosphate (12H2O)
2.5 g/L
Features

Key features

  • 01

    Staphylococcus aureus enrichment

    GF1013F is stated for enrichment of S. aureus and culture of fastidious organisms.

  • 02

    Pure culture option

    KL1013F is stated for pure culture of S. aureus and nutritionally demanding bacteria.

  • 03

    ISO 6888 reference

    Listed for the dehydrated powder GF1013F.

  • 04

    Different formulations by form

    Powder uses calf brain and beef heart infusions; granulated uses tryptone and beef heart powder.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.