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Reference: GF1023F / KL1023F

Columbia Blood Agar Base

Nutrient-rich base for fastidious microorganisms; with defibrinated sheep blood added it shows hemolytic reactions.

Key specifications

Product code (dehydrated)
GF1023F
Product code (granulated)
KL1023F
Form
Dehydrated powder (GF1023F) and granulated (KL1023F)
Standard (ACC)
ISO 10271 (GF1023F page)
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Columbia Blood Agar Base is a peptic-digest agar base intended for the cultivation of fastidious microorganisms and for defining hemolytic reactions. Biocomma supplies it as a dehydrated powder (GF1023F, which cites ISO 10271) and as a granulated medium (KL1023F).

The base is made up at 41.0 g per litre of purified water, boiled and autoclaved at 121 °C for 15 minutes. After cooling to 45-50 °C, 5% sterile defibrinated sheep blood is added. Final pH is 7.4±0.2 and shelf life of the sealed product is 3 years.

Specifications

Technical specifications

13 parameters

Identification

Product code (dehydrated)
GF1023F
Product code (granulated)
KL1023F
Form
Dehydrated powder (GF1023F) and granulated (KL1023F)
Standard (ACC)
ISO 10271 (GF1023F page)

Preparation

Preparation
Suspend 41.0 g in 1 L of purified water; boil to dissolve; autoclave at 121 °C for 15 minutes; cool to 45-50 °C and add 5% sterile defibrinated sheep blood

Properties

Final pH (25 °C)
7.4±0.2

Storage & handling

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use.
Shelf life (sealed product)
3 years
Precautions
Not for clinical use. Do not use if the powder is lumpy. After use, autoclave all contaminated materials at 121 °C for 30 minutes before disposal.

Composition

Peptic Digest of Animal Tissue
23.0 g/L
Starch
1.0 g/L
Sodium Chloride
5.0 g/L
Agar
12.0 g/L
Features

Key features

  • 01

    Fastidious organisms

    Intended for cultivating fastidious microorganisms.

  • 02

    Hemolysis reactions

    Used with 5% sterile defibrinated sheep blood to define hemolytic reactions.

  • 03

    Powder or granulated

    Available as dehydrated powder GF1023F or granulated KL1023F at the same 41.0 g/L.

  • 04

    Three-year shelf life

    Sealed product keeps for 3 years when stored dark and dry at 2-25 °C.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.