Copure SPE (HLB family)
Biocomma Copure HLB: PS/DVB copolymer, pH 0–14, 500–750 m²/g, 200–400 Å, cartridges from 30 mg/1 mL to 500 mg/12 mL.
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Reference: HCN536 / HAMH434
CommaSil® CN HPLC Column
A cyano-bonded HPLC column usable in normal-phase, reversed-phase and HILIC modes, with selectivity different from C18, C8 and phenyl phases; also lists a CommaSil® AMIDE-H column for sugars.
Key specifications
- Phase
- Cyano (CN) bonded phase with propyl chain
- Separation modes
- Normal phase, reversed phase and HILIC
- CN column
- 5 μm, 250 × 4.6 mm; catalog number HCN536
- AMIDE-H sugar column
- 3 μm, 4.6 mm × 150 mm; catalog number HAMH434
- Configured to your application by our specialists
- Installed and commissioned on site
- Training and after-sales support across Jordan, the GCC and MENA
CommaSil® CN HPLC Column
Request a quoteOverview
Product overview
CommaSil® CN works in three separation modes - normal phase, reversed phase and HILIC - giving multiple retention mechanisms. The surface-bonded cyano group interacts strongly with polar compounds through dipole-dipole interactions, while the propyl chain adds hydrophobic interaction. This combination gives unique selectivity that broadens the range of chromatographic applications.
The column elutes hydrophobic molecules quickly, has distinctive selectivity for polar compounds, and gives good peak shape for strongly basic compounds, including ammonium salts. Its selectivity differs from common C18, C8, C4 and phenyl reversed-phase columns, and it can be considered when C18 or C8 columns do not effectively elute extremely hydrophobic compounds.
The page shows azocarbonamide determination on the CN column (HCN536) and a sugar separation on the CommaSil® AMIDE-H column (HAMH434), which is listed in the same ordering table.
Specifications
Technical specifications
6 parameters
CommaSil® CN
- Phase
- Cyano (CN) bonded phase with propyl chain
- Separation modes
- Normal phase, reversed phase and HILIC
Ordering information
- CN column
- 5 μm, 250 × 4.6 mm; catalog number HCN536
- AMIDE-H sugar column
- 3 μm, 4.6 mm × 150 mm; catalog number HAMH434
Application example
- Example: azocarbonamide
- 250 × 4.6 mm, 5 μm (HCN536); UV 245 nm; 0.6 mL/min; 25 °C; 10 μL; 20 mmol/L ammonium acetate
- Example: sugars on AMIDE-H
- 4.6 × 150 mm, 3 μm (HAMH434); ELSD; 1.0 mL/min; 50 °C; sorbitol, fructose, glucose, sucrose, lactose resolved
Features
Key features
- 01
Three separation modes
Normal phase, reversed phase and HILIC.
- 02
Distinct selectivity
Differs from C18, C8, C4 and phenyl reversed-phase columns.
- 03
Polar-compound selectivity
Strong dipole-dipole interaction of the cyano group with polar compounds.
- 04
Good peak shape for bases
Good peak shape for strongly basic compounds, including ammonium salts.
- 05
AMIDE-H sugar column listed
The page also lists CommaSil® AMIDE-H (HAMH434) for sugar separation.
From order to operation
We stay with the instrument after it arrives
Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.
- 01
Consult
- 02
Plan
- 03
Supply
- 04
Install
- 05
Train
- 06
Support
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Talk to the technical team
Tell us the application. We will specify the system.
Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.