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Al-Shaker for Scientific and Medical Supplies

Reference: GF1017F

CT-Sorbitol MacConkey Agar Base

Sorbitol-based MacConkey agar base for the isolation of Escherichia coli O157:H7, used with supplement TJ030F.

Key specifications

Product code
GF1017F
Required supplement
TJ030F (one vial per 200 mL base)
Form
Dehydrated culture medium (powder)
Preparation
Suspend 51.5 g in 1 L of purified water; boil to dissolve; autoclave at 121 °C for 15 minutes; cool to below 50 °C and aseptically add one vial of supplement TJ030F to every 200 mL of base medium
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

CT-Sorbitol MacConkey Agar Base is a dehydrated base medium listed for the isolation of Escherichia coli O157:H7. In place of lactose, it uses sorbitol as the carbohydrate, with bile salts, neutral red and crystal violet in the formulation.

The powder is prepared at 51.5 g per litre of purified water and autoclaved at 121 °C for 15 minutes. After cooling below 50 °C, one vial of supplement TJ030F is aseptically added to every 200 mL of base. Final pH is 7.2±0.2 and the sealed product has a 3-year shelf life.

Specifications

Technical specifications

15 parameters

Identification

Product code
GF1017F
Required supplement
TJ030F (one vial per 200 mL base)
Form
Dehydrated culture medium (powder)

Preparation

Preparation
Suspend 51.5 g in 1 L of purified water; boil to dissolve; autoclave at 121 °C for 15 minutes; cool to below 50 °C and aseptically add one vial of supplement TJ030F to every 200 mL of base medium

Properties

Final pH (25 °C)
7.2±0.2

Storage & handling

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use.
Shelf life (sealed product)
3 years
Precautions
Not for clinical use. Do not use if the powder is lumpy. After use, autoclave all contaminated materials at 121 °C for 30 minutes before disposal.

Composition

Peptone
20.0 g/L
Sorbitol
10.0 g/L
Sodium Chloride
5.0 g/L
Bile Salts No. 3
1.5 g/L
Neutral Red
0.03 g/L
Crystal Violet
0.001 g/L
Agar
15.0 g/L
Features

Key features

  • 01

    E. coli O157:H7 isolation

    Listed for the isolation of Escherichia coli O157:H7.

  • 02

    Sorbitol as carbohydrate

    Sorbitol (10.0 g/L) replaces lactose in the MacConkey-type formulation.

  • 03

    Supplement required

    Add one vial of TJ030F to every 200 mL of base after cooling below 50 °C.

  • 04

    Three-year shelf life

    Sealed product keeps for 3 years when stored dark and dry at 2-25 °C.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

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