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Reference: GF1130F / KL1130F

Deoxycholate Hydrogen Sulfide Lactose Agar (DHL Agar)

Selective plating agar for separating Salmonella, using lactose, sucrose, deoxycholate, thiosulfate and neutral red; no autoclaving required.

Key specifications

Product code (dehydrated powder)
GF1130F
Product code (granulated)
KL1130F
Form
Dehydrated powder (GF) and granulated (KL) versions
Quantity per litre of distilled or deionized water
63.3 g
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Deoxycholate Hydrogen Sulfide Lactose Agar is used for the selective separation of Salmonella. The formula includes lactose and sucrose as carbohydrates, sodium deoxycholate and sodium citrate as selective agents, sodium thiosulfate with ammonium ferric citrate for sulfide detection, and neutral red as the pH indicator, in a 15 g/L agar base.

Suspend 63.3 g in 1 L of distilled or deionized water and boil with frequent agitation until dissolved. No autoclaving is required: cool to about 50 °C, pour into sterile plates and let them set. The final pH is 7.3 ± 0.2.

Dehydrated powder (GF1130F) and granulated (KL1130F) versions share one composition. The product is not for clinical use.

Specifications

Technical specifications

19 parameters

Identification

Product code (dehydrated powder)
GF1130F
Product code (granulated)
KL1130F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of distilled or deionized water
63.3 g
Dissolution
Heat with frequent agitation and boil to dissolve completely
Pouring
No autoclaving needed; cool to about 50 °C, pour into sterile plates and allow to solidify
Final pH (25 °C)
7.3 ± 0.2

Storage

Storage
Store in a dry place away from light; tighten the cap immediately after use
Shelf life (sealed)
3 years

Composition

Peptone
20 g/L
Beef Paste Powder
3 g/L
Lactose
10 g/L
Sucrose
10 g/L
Sodium Deoxycholate
1 g/L
Sodium Thiosulfate
2.3 g/L
Sodium Citrate
1 g/L
Ammonium Ferric Citrate
1 g/L
Neutral Red
0.03 g/L
Agar
15 g/L
Features

Key features

  • 01

    Salmonella separation

    Used for the selective separation of Salmonella.

  • 02

    Sulfide and carbohydrate system

    Lactose 10, sucrose 10, sodium thiosulfate 2.3 and ammonium ferric citrate 1 g/L.

  • 03

    Neutral red indicator

    Neutral red 0.03 g/L with sodium deoxycholate 1 g/L.

  • 04

    No autoclave step

    Boil to dissolve, cool to about 50 °C and pour.

  • 05

    Shelf life

    3 years sealed, dry and away from light.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.