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Reference: GF1031F / KL1031F

EC Broth

Lactose broth for detecting coliform bacteria at 35 °C and Escherichia coli at an elevated temperature of 44.5 or 45 °C.

Key specifications

Product code (dehydrated powder)
GF1031F
Product code (granulated)
KL1031F
Form
Dehydrated powder and granulated forms
Preparation
Suspend 37.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; dispense 8 mL per test tube containing small glass tubes; autoclave at 121 °C for 15 min.
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

EC Broth is used for the detection of coliform bacteria at 35 °C and of Escherichia coli at an elevated temperature (44.5 or 45 °C). Tryptone, lactose, bile salts No.3 and phosphate buffers make up the formulation, with a final pH of 6.9±0.1.

Suspend 37.0 g in 1 L of purified water and boil with frequent agitation to dissolve. Dispense 8 mL per test tube containing small glass tubes and autoclave at 121 °C for 15 minutes. Dehydrated powder (GF1031F) has a 5-year sealed shelf life and the granulated form (KL1031F) 3 years, both stored dry and away from light.

Specifications

Technical specifications

15 parameters

Product

Product code (dehydrated powder)
GF1031F
Product code (granulated)
KL1031F
Form
Dehydrated powder and granulated forms

Preparation

Preparation
Suspend 37.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; dispense 8 mL per test tube containing small glass tubes; autoclave at 121 °C for 15 min.
Final pH (25 °C)
6.9±0.1

Storage

Storage
Store in a dry place away from light; tighten the cap immediately after use.
Shelf life (sealed) (dehydrated powder)
5 years
Shelf life (sealed) (granulated)
3 years

Composition

Tryptone
20 g/L
Lactose
5 g/L
Sodium chloride
5 g/L
Dipotassium hydrogen phosphate
4 g/L
Potassium dihydrogen phosphate
1.5 g/L
Bile salts No.3
1.5 g/L

Application

Incubation temperature stated in application
35 °C for coliforms; 44.5 or 45 °C for Escherichia coli
Features

Key features

  • 01

    Coliform detection at 35 °C

    Published for detecting coliform bacteria at 35 °C.

  • 02

    E. coli at elevated temperature

    Used at 44.5 or 45 °C for Escherichia coli.

  • 03

    Tube format

    8 mL per test tube with small glass tubes.

  • 04

    Two forms

    Dehydrated powder GF1031F (5 years) and granulated KL1031F (3 years).

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.