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Reference: GF1182F / KL1182F

Fraser Broth (FB1/FB2) Base

Base broth for the selective enrichment of Listeria monocytogenes, completed with the FB1 or FB2 supplements; available as powder or granulated medium.

Key specifications

Product code (dehydrated powder)
GF1182F
Product code (granulated)
KL1182F
Form
Dehydrated powder (GF) and granulated (KL) versions
Standards (ACC field)
ISO (KL1182F application statement)
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Fraser Broth (FB1/FB2) Base is used for the selective enrichment of Listeria monocytogenes, and the granulated listing notes ISO. The base contains enzymatic digests of animal tissues and casein, meat extract, yeast extract, 20.0 g/L sodium chloride, a phosphate buffer and 1.0 g/L aesculin.

Suspend 57.4 g in 1 L of purified water, heat with frequent agitation until dissolved, autoclave at 121 °C for 15 minutes and cool to 50 °C. For FB1, aseptically add one set (A+B) of supplement TJ049F per 225 mL of base. For FB2, add one set of supplements TJ071F and TJ072F per 100 mL of base. The final pH is 7.2 ± 0.2.

Powder (GF1182F) and granulated (KL1182F) versions share one composition and a 5-year sealed shelf life, stored in a dark, dry place at 2-25 °C. The product is not for clinical use.

Specifications

Technical specifications

19 parameters

Identification

Product code (dehydrated powder)
GF1182F
Product code (granulated)
KL1182F
Form
Dehydrated powder (GF) and granulated (KL) versions
Standards (ACC field)
ISO (KL1182F application statement)

Preparation

Quantity per litre of purified water
57.4 g
Preparation
Heat with frequent agitation to dissolve completely; autoclave at 121 °C for 15 minutes and cool to 50 °C
FB1 supplementation
Aseptically add one set (A+B) of supplement (Cat. TJ049F) per 225 mL base medium
FB2 supplementation
Aseptically add one set of supplement (Cat. TJ071F and Cat. TJ072F) per 100 mL base medium
Final pH (25 °C)
7.2 ± 0.2

Storage

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use
Shelf life (sealed)
5 years

Composition

Enzymatic Digest of Animal Tissues
5.0 g/L
Enzymatic Digest of Casein
5.0 g/L
Meat Extract
5.0 g/L
Yeast Extract
5.0 g/L
Sodium Chloride
20.0 g/L
Disodium Hydrogen Phosphate Dihydrate
12.0 g/L
Potassium Dihydrogen Phosphate
1.35 g/L
Aesculin
1.0 g/L
Features

Key features

  • 01

    Listeria monocytogenes enrichment

    For selective enrichment of Listeria monocytogenes.

  • 02

    FB1 and FB2 supplementation

    FB1: TJ049F per 225 mL; FB2: TJ071F and TJ072F per 100 mL.

  • 03

    Aesculin-containing base

    Aesculin 1.0 g/L with sodium chloride 20.0 g/L and a phosphate buffer.

  • 04

    Two forms

    Dehydrated powder (GF1182F) or granulated (KL1182F).

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.