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Reference: GF1030F / KL1030F

Gram Negative Enrichment Broth

Enrichment broth for Gram-negative Enterobacteriaceae, especially Shigella and Proteus, supplied as a dehydrated powder or in granulated form.

Key specifications

Product code (dehydrated powder)
GF1030F
Product code (granulated)
KL1030F
Form
Dehydrated powder (GF) and granulated (KL) versions
Quantity per litre of distilled or deionized water
39 g
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Gram Negative Enrichment Broth is a tryptone-based liquid medium used to enrich Gram-negative Enterobacteriaceae, with Shigella and Proteus named as the target organisms. Glucose, mannitol and a phosphate and citrate system provide nutrients and buffering, while sodium deoxycholate is included in the formula.

The medium is prepared at 39 g per litre of distilled or deionized water, heated to boiling until fully dissolved, dispensed into flasks at 225 mL each and autoclaved at 115 °C for 15 minutes. The final pH is 7.0 ± 0.2.

It is offered both as a dehydrated powder (GF1030F) and a granulated version (KL1030F) with the same published composition. It is not intended for clinical use.

Specifications

Technical specifications

17 parameters

Identification

Product code (dehydrated powder)
GF1030F
Product code (granulated)
KL1030F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of distilled or deionized water
39 g
Dissolution
Stir and heat to boiling until completely dissolved
Dispensing and sterilisation
Dispense 225 mL per flask; autoclave at 115 °C for 15 minutes
Final pH (25 °C)
7.0 ± 0.2

Storage

Storage
Store in a dark, dry place at room temperature; tighten the cap immediately after use
Shelf life (sealed)
3 years

Composition

Tryptone
20.0 g/L
Glucose
1.0 g/L
Potassium Dihydrogen Phosphate
1.5 g/L
Dipotassium Hydrogen Phosphate
4.0 g/L
Mannitol
2.0 g/L
Sodium Citrate
5.0 g/L
Sodium Deoxycholate
0.5 g/L
Sodium Chloride
5.0 g/L
Features

Key features

  • 01

    Targeted enrichment

    Used for the enrichment of Gram-negative Enterobacteriaceae, specifically Shigella and Proteus.

  • 02

    Defined composition

    Tryptone 20 g/L with glucose, mannitol, phosphate and citrate buffering and sodium deoxycholate 0.5 g/L.

  • 03

    Gentle sterilisation

    Autoclaved at 115 °C for 15 minutes in 225 mL flask portions.

  • 04

    Two physical forms

    Available as dehydrated powder or granulated medium.

  • 05

    Simple storage

    Keep in a dark, dry place at room temperature with the cap tightly closed.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.