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Al-Shaker for Scientific and Medical Supplies

Reference: GF1055F

King's B Medium

Non-selective agar for isolating and cultivating Pseudomonas species and promoting pigment production, prepared as slants.

Key specifications

Product code
GF1055F
Form
Dehydrated culture medium (powder)
Standard (ACC)
ISO 16266
Preparation
Suspend 38.0 g in 1 L of purified water and add 10 mL glycerol; boil to dissolve; distribute into test tubes; autoclave at 121 °C for 15 minutes; cool tubes in a slanted position to form slants
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Overview

Product overview

King's B Medium is listed for non-selective isolation, cultivation and pigment production of Pseudomonas species, and the product page cites ISO 16266. The base is built on peptone with dipotassium hydrogen phosphate and magnesium sulfate, and glycerol is added at preparation.

Suspend 38.0 g in 1 L of purified water with 10 mL of glycerol, boil to dissolve, dispense into test tubes and autoclave at 121 °C for 15 minutes. The tubes are then cooled in a slanted position to give slants. Sealed product has a 3-year shelf life.

Specifications

Technical specifications

13 parameters

Identification

Product code
GF1055F
Form
Dehydrated culture medium (powder)
Standard (ACC)
ISO 16266

Preparation

Preparation
Suspend 38.0 g in 1 L of purified water and add 10 mL glycerol; boil to dissolve; distribute into test tubes; autoclave at 121 °C for 15 minutes; cool tubes in a slanted position to form slants

Properties

Final pH (25 °C)
7.2±0.2

Storage & handling

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use.
Shelf life (sealed product)
3 years
Precautions
Not for clinical use. Do not use if the powder is lumpy. After use, autoclave all contaminated materials at 121 °C for 30 minutes before disposal.

Composition

Peptone
20.0 g/L
Dipotassium Hydrogen Phosphate
1.5 g/L
Magnesium Sulfate Heptahydrate
1.5 g/L
Agar
15.0 g/L
Glycerol (added at preparation)
10 mL/L
Features

Key features

  • 01

    Pseudomonas cultivation

    For non-selective isolation, cultivation and pigment production of Pseudomonas species.

  • 02

    ISO 16266 reference

    The product page lists ISO 16266 as its standard reference.

  • 03

    Slant preparation

    Dispensed into test tubes, autoclaved and cooled in a slanted position.

  • 04

    Glycerol added at preparation

    10 mL of glycerol per litre is added when suspending the powder.

From order to operation

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  2. 02

    Plan

  3. 03

    Supply

  4. 04

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  5. 05

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  6. 06

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