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Reference: GF1256F / GF1254F

LB Agar (Lennox)

Dehydrated Lennox-formula LB agar for E. coli culture, offered as a general-purpose pH 7.0 version and a pH 7.5 version for cloning and expression work.

Key specifications

Product code (pH 7.0)
GF1256F (500 g/bottle); GF1256EF (10 kg/bucket)
Product code (pH 7.5)
GF1254F (500 g/bottle); GF1254EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 35.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

LB Agar (Lennox) is used for cultivating Escherichia coli in molecular biology experiments. The pH 7.0 version suits general bacterial culture, strain maintenance and routine laboratory use, while the pH 7.5 version is optimised for molecular cloning, plasmid preparation, high-density E. coli culture and protein expression.

Tryptone and yeast extract provide nitrogen sources, vitamins and growth factors, sodium chloride maintains osmotic balance and agar sets the medium. Both versions are prepared at 35.0 g per litre of purified water and autoclaved.

Specifications

Technical specifications

11 parameters

General

Product code (pH 7.0)
GF1256F (500 g/bottle); GF1256EF (10 kg/bucket)
Product code (pH 7.5)
GF1254F (500 g/bottle); GF1254EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 35.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
Final pH (25 °C), standard version
7.0 ±0.2
Final pH (25 °C), pH 7.5 version
7.5 ±0.2
Shelf life
3 years

Composition

Tryptone (pH 7.0 version)
10 g/L
Yeast Extract (pH 7.0 version)
5 g/L
Sodium Chloride (pH 7.0 version)
5 g/L
Agar (pH 7.0 version)
15 g/L
Features

Key features

  • 01

    Two pH grades

    pH 7.0 (GF1256F) for routine culture; pH 7.5 (GF1254F) for cloning, plasmid prep and expression.

  • 02

    Molecular biology standard

    Used for E. coli cultivation in molecular biology experiments.

  • 03

    Same preparation

    35.0 g per litre of purified water, autoclaved at 121 °C for 20 minutes.

  • 04

    Pack sizes

    500 g bottle or 10 kg bucket; 3-year shelf life.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.