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Reference: GF1251F / GF1167F

LB Agar (Miller)

Dehydrated Miller-formula LB agar (10 g/L NaCl) for E. coli culture, offered at pH 7.0 for general use and pH 7.5 for cloning and expression.

Key specifications

Product code (pH 7.0)
GF1251F (500 g/bottle); GF1251EF (10 kg/bucket)
Product code (pH 7.5)
GF1167F (500 g/bottle); GF1167EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 40.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

LB Agar (Miller) is used to cultivate Escherichia coli in molecular biology experiments. The pH 7.0 version is intended for general bacterial culture, strain maintenance and routine use; the pH 7.5 version is optimised for molecular cloning, plasmid preparation, high-density E. coli culture and protein expression.

Both versions share the same composition: tryptone, yeast extract, 10 g/L sodium chloride and agar. Suspend 40.0 g per litre of purified water, boil to dissolve and autoclave.

Specifications

Technical specifications

11 parameters

General

Product code (pH 7.0)
GF1251F (500 g/bottle); GF1251EF (10 kg/bucket)
Product code (pH 7.5)
GF1167F (500 g/bottle); GF1167EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 40.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
Final pH (25 °C), standard version
7.0 ±0.2
Final pH (25 °C), pH 7.5 version
7.5 ±0.2
Shelf life
3 years

Composition

Tryptone
10 g/L
Yeast Extract
5 g/L
Sodium Chloride
10 g/L
Agar
15 g/L
Features

Key features

  • 01

    Two pH grades

    pH 7.0 (GF1251F) for routine work; pH 7.5 (GF1167F) for cloning, plasmid prep and expression.

  • 02

    Miller salt level

    Contains 10 g/L sodium chloride.

  • 03

    Simple preparation

    40.0 g per litre of purified water, autoclaved at 121 °C for 20 minutes.

  • 04

    Pack sizes

    500 g bottle or 10 kg bucket; 3-year shelf life.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.