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Reference: GF1181F / GF1252F

LB Broth (Miller)

Dehydrated Miller-formula LB broth (10 g/L NaCl) for E. coli culture, offered at pH 7.0 for general use and pH 7.5 for cloning and expression.

Key specifications

Product code (pH 7.0)
GF1181F (500 g/bottle); GF1181EF (10 kg/bucket); GF1181HF (25 kg/drum); GF1181KF (1 L pouch, 10 pouches/box)
Product code (pH 7.5)
GF1252F (500 g/bottle); GF1252EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 25.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

LB Broth (Miller) is used for cultivating Escherichia coli in molecular biology experiments, following Molecular Cloning: A Laboratory Manual, 4th Edition. The pH 7.0 version suits general bacterial culture, strain maintenance and routine use; the pH 7.5 version is optimised for molecular cloning, plasmid preparation, high-density culture and protein expression.

Both versions contain 10 g/L tryptone, 5 g/L yeast extract and 10 g/L sodium chloride. Suspend 25.0 g per litre of purified water, boil to dissolve and autoclave.

Specifications

Technical specifications

11 parameters

General

Product code (pH 7.0)
GF1181F (500 g/bottle); GF1181EF (10 kg/bucket); GF1181HF (25 kg/drum); GF1181KF (1 L pouch, 10 pouches/box)
Product code (pH 7.5)
GF1252F (500 g/bottle); GF1252EF (10 kg/bucket)
Form
Dehydrated powder
Preparation (both versions)
Suspend 25.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
Final pH (25 °C), standard version
7.0 ±0.2
Final pH (25 °C), pH 7.5 version
7.5 ±0.2
Reference
Molecular Cloning: A Laboratory Manual, 4th Edition
Shelf life
3 years

Composition

Tryptone
10 g/L
Yeast Extract
5 g/L
Sodium Chloride
10 g/L
Features

Key features

  • 01

    Two pH grades

    pH 7.0 (GF1181F) for routine work; pH 7.5 (GF1252F) for cloning, plasmid prep and expression.

  • 02

    Miller salt level

    10 g/L sodium chloride.

  • 03

    Simple preparation

    25.0 g per litre of purified water, autoclaved at 121 °C for 20 minutes.

  • 04

    Several pack sizes

    500 g bottle, 10 kg bucket, and for the pH 7.0 grade also a 25 kg drum or 1 L pouch.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.