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Reference: GF1033F / KL1033F

Modified EC Broth

EC-type broth for the selective enrichment of enterohemorrhagic Escherichia coli O157:H7, completed with filter-sterilized novobiocin at 20 mg/L.

Key specifications

Product code (dehydrated powder)
GF1033F
Product code (granulated)
KL1033F
Form
Dehydrated powder and granulated forms
Preparation
Weigh 36.62 g and dissolve in 1000 mL of distilled water; autoclave at 121 °C for 15 min; after cooling to below 50 °C add filter-sterilized novobiocin solution to a final concentration of 20 mg/L.
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Modified EC Broth is used for the selective enrichment of enterohemorrhagic Escherichia coli O157:H7. The base contains tryptone (20.0 g/L), lactose, a reduced level of bile salt No. 3 (1.1 g/L), phosphate buffers and sodium chloride, with a final pH of 6.9±0.2.

Weigh 36.62 g into 1000 mL of distilled water and autoclave at 121 °C for 15 minutes. After cooling to below 50 °C, add filter-sterilized novobiocin solution to reach a final concentration of 20 mg/L. Dehydrated powder (GF1033F) and granulated (KL1033F) forms are stored in a dark, dry place at room temperature and have a 3-year sealed shelf life.

Specifications

Technical specifications

14 parameters

Product

Product code (dehydrated powder)
GF1033F
Product code (granulated)
KL1033F
Form
Dehydrated powder and granulated forms

Preparation

Preparation
Weigh 36.62 g and dissolve in 1000 mL of distilled water; autoclave at 121 °C for 15 min; after cooling to below 50 °C add filter-sterilized novobiocin solution to a final concentration of 20 mg/L.
Final pH (25 °C)
6.9±0.2
Novobiocin added at preparation
20 mg/L

Storage

Storage
Store in a dark and dry place at room temperature; tighten the bottle cap immediately after use.
Shelf life (sealed)
3 years

Composition

Tryptone
20.0 g/L
Lactose
5.0 g/L
Bile salt No. 3
1.1 g/L
Dipotassium hydrogen phosphate
4.0 g/L
Potassium dihydrogen phosphate
1.5 g/L
Sodium chloride
5.0 g/L
Features

Key features

  • 01

    E. coli O157:H7 enrichment

    Published for selective enrichment of enterohemorrhagic E. coli O157:H7.

  • 02

    Novobiocin supplementation

    Add filter-sterilized novobiocin to 20 mg/L once the medium is below 50 °C.

  • 03

    Defined preparation

    36.62 g in 1000 mL of distilled water, autoclaved at 121 °C for 15 minutes.

  • 04

    Two forms

    Dehydrated powder GF1033F and granulated KL1033F.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.