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Reference: GF1041F / KL1041F

Modified Lauryl Sulfate Tryptone Broth Base (mLST)

Base broth for the selective enrichment of Cronobacter (Enterobacter sakazakii), with a supporting reagent added after sterilisation.

Key specifications

Product code (dehydrated powder)
GF1041F
Product code (granulated)
KL1041F
Form
Dehydrated powder (GF) and granulated (KL) versions
Quantity per litre of distilled or deionized water
64.6 g
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Modified Lauryl Sulfate Tryptone Broth Base is used for the selective enrichment of Cronobacter, also called Enterobacter sakazakii. The formula combines 20 g/L tryptone with a high sodium chloride level (34 g/L), lactose, a phosphate buffer and 0.1 g/L sodium lauryl sulfate.

Suspend 64.6 g in 1 L of distilled or deionized water, boil with frequent agitation until dissolved and autoclave at 121 °C for 15 minutes. Cool the medium to about 30 °C, add one supporting reagent per 100 mL of basic culture medium, mix and set aside. The final pH is 6.8 ± 0.2.

Powder (GF1041F) and granulated (KL1041F) versions share one composition and a 3-year sealed shelf life. Not for clinical use.

Specifications

Technical specifications

15 parameters

Identification

Product code (dehydrated powder)
GF1041F
Product code (granulated)
KL1041F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of distilled or deionized water
64.6 g
Dissolution
Heat with frequent agitation and boil to dissolve completely
Sterilisation
Autoclave at 121 °C for 15 minutes
Supplementation
Cool to about 30 °C; add 1 supporting reagent per 100 mL basic culture medium and mix
Final pH (25 °C)
6.8 ± 0.2

Storage

Storage
Store in a dry place away from light; tighten the cap immediately after use
Shelf life (sealed)
3 years

Composition

Tryptone
20 g/L
Sodium Chloride
34 g/L
Lactose
5 g/L
Potassium Dihydrogen Phosphate
2.75 g/L
Sodium Lauryl Sulfate
0.1 g/L
Features

Key features

  • 01

    Cronobacter enrichment

    Used for the selective enrichment of Cronobacter (Enterobacter sakazakii).

  • 02

    Supporting reagent

    One supporting reagent per 100 mL of base is added after cooling to about 30 °C.

  • 03

    Defined formula

    Tryptone 20, sodium chloride 34, lactose 5, potassium dihydrogen phosphate 2.75 and sodium lauryl sulfate 0.1 g/L.

  • 04

    Two forms

    Powder (GF1041F) or granulated (KL1041F).

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.