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Al-Shaker for Scientific and Medical Supplies

Reference: GF1311F

Modified Semisolid Rappaport Vassiliadis Medium (MSRV) Base

Base for semi-solid MSRV medium used to detect motile Salmonella in food and environmental samples; completed with a novobiocin supplement. ISO 6579 and ISO 11133 listed.

Key specifications

Product code (dehydrated powder)
GF1311F
Form
Dehydrated powder, base (supplement required)
Standards (ACC field)
ISO 6579, ISO 11133
Quantity per litre of purified water
31.6 g
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Modified Semisolid Rappaport Vassiliadis Medium (MSRV) Base is the base version of MSRV, used for the detection of motile Salmonella species from food and environmental samples. It shares the MSRV formula (2.7 g/L agar, 10.9 g/L anhydrous magnesium chloride, 0.04 g/L malachite green oxalate) but is supplied without novobiocin, which is added from a supplement. The source lists ISO 6579 and ISO 11133 as standards.

Suspend 31.6 g in 1 L of purified water, heat with frequent agitation and boil until dissolved, avoiding overheating, and do not autoclave. Cool to about 50 °C, add one tube of supplement (5 mg novobiocin sodium salt) per 500 mL of base medium, mix well and pour into sterile Petri dishes. The final pH is 5.1-5.4.

Sealed shelf life is 3 years, stored in a dark, dry place at 2-25 °C. Not for clinical use.

Specifications

Technical specifications

16 parameters

Identification

Product code (dehydrated powder)
GF1311F
Form
Dehydrated powder, base (supplement required)
Standards (ACC field)
ISO 6579, ISO 11133

Preparation

Quantity per litre of purified water
31.6 g
Dissolution
Heat with frequent agitation and boil to dissolve completely; avoid overheating; do not autoclave
Supplementation and pouring
Cool to about 50 °C; add 1 tube of supplement (5 mg novobiocin sodium salt) per 500 mL base medium, mix well and pour into sterile Petri dishes
Final pH (25 °C)
5.1-5.4

Storage

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use
Shelf life (sealed)
3 years

Composition

Enzymatic digest of animal and plant tissue
4.6 g/L
Acid hydrolysate of casein
4.6 g/L
Sodium chloride
7.3 g/L
Potassium dihydrogen phosphate
1.5 g/L
Magnesium chloride anhydrous
10.9 g/L
Malachite green oxalate
0.04 g/L
Agar
2.7 g/L
Features

Key features

  • 01

    Motile Salmonella detection

    Used for the detection of motile Salmonella species from food and environmental samples.

  • 02

    Supplement dosing

    One tube of supplement (5 mg novobiocin sodium salt) per 500 mL of base.

  • 03

    Standards listed

    ISO 6579 and ISO 11133.

  • 04

    No autoclaving

    Avoid overheating and do not autoclave the medium.

  • 05

    Storage

    Dark, dry place at 2-25 °C; 3-year sealed shelf life.

From order to operation

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  1. 01

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  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

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  6. 06

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