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Reference: GF1025F / GF630F / KL1025F

Nutrient Broth (NB)

General-purpose liquid medium for cultivating less fastidious microorganisms, made from peptone, beef extract and sodium chloride.

Key specifications

Product code (dehydrated powder)
GF1025F
Product code (dehydrated powder, pH 7.2 formula)
GF630F
Product code (granulated)
KL1025F
Form
Dehydrated powder (GF) and granulated (KL) versions
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Nutrient Broth (NB) is a general-purpose liquid medium for the cultivation of less fastidious microorganisms. Its formula is limited to 10.0 g/L peptone, 3.0 g/L beef extract and 5.0 g/L sodium chloride, with no selective agents.

Suspend 18.0 g in 1 L of purified water, boil with frequent agitation until dissolved, then distribute and autoclave at 121 °C for 15 minutes. The final pH is 7.4 ± 0.2 for GF1025F and KL1025F, and 7.2 ± 0.2 for GF630F.

Three catalogue codes are listed: GF1025F and GF630F (dehydrated powder) and KL1025F (granulated). All have a 5-year sealed shelf life and are stored in a dark, dry place at 2-25 °C. The product is not for clinical use.

Specifications

Technical specifications

13 parameters

Identification

Product code (dehydrated powder)
GF1025F
Product code (dehydrated powder, pH 7.2 formula)
GF630F
Product code (granulated)
KL1025F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of purified water
18.0 g
Preparation
Heat with frequent agitation and boil to dissolve completely; distribute and autoclave at 121 °C for 15 minutes
Final pH (25 °C), GF1025F and KL1025F
7.4 ± 0.2
Final pH (25 °C), GF630F
7.2 ± 0.2

Storage

Storage
Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use
Shelf life (sealed)
5 years

Composition

Peptone
10.0 g/L
Beef Extract
3.0 g/L
Sodium Chloride
5.0 g/L
Features

Key features

  • 01

    General cultivation

    Used for the general cultivation of less fastidious microorganisms.

  • 02

    Simple formula

    Peptone 10.0, beef extract 3.0 and sodium chloride 5.0 g/L.

  • 03

    Standard preparation

    18.0 g per litre, autoclaved at 121 °C for 15 minutes.

  • 04

    Long shelf life

    5 years sealed, stored dark and dry at 2-25 °C.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.