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Reference: GF1083F / KL1083F

Peptone Yeast Glucose (PYG) Broth

Broth for colony counting of lactic acid bacteria in fermented beverages, supplemented before use with vitamin K1 and hemin chloride solutions.

Key specifications

Product code (dehydrated powder)
GF1083F
Product code (granulated)
KL1083F
Form
Dehydrated powder and granulated forms
Preparation
Weigh 36 g into 1 L of distilled or deionized water; stir and heat to boiling until dissolved; dispense into flasks and autoclave at 121 °C for 15 min; before use add one vial of vitamin K1 solution and one vial of hemin chloride solution per 100 mL of sterilized medium.
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Peptone Yeast Glucose (PYG) Broth is used for the colony counting of lactic acid bacteria in fermented beverages. The medium is built on peptone, glucose and yeast extract with phosphate, bicarbonate and trace mineral salts, and has a final pH of 6.1±0.2.

Weigh 36 g into 1 L of distilled or deionized water, heat to boiling until dissolved and autoclave at 121 °C for 15 minutes. Before use, one vial of vitamin K1 solution and one vial of hemin chloride solution are added to every 100 mL of sterilized medium. Available as dehydrated powder (GF1083F) and granulated (KL1083F), each with a 3-year sealed shelf life.

Specifications

Technical specifications

16 parameters

Product

Product code (dehydrated powder)
GF1083F
Product code (granulated)
KL1083F
Form
Dehydrated powder and granulated forms

Preparation

Preparation
Weigh 36 g into 1 L of distilled or deionized water; stir and heat to boiling until dissolved; dispense into flasks and autoclave at 121 °C for 15 min; before use add one vial of vitamin K1 solution and one vial of hemin chloride solution per 100 mL of sterilized medium.
Final pH (25 °C)
6.1±0.2

Storage

Storage
Store in a dark and dry place at room temperature; tighten the bottle cap immediately after use.
Shelf life (sealed)
3 years

Composition

Peptone
20 g/L
Glucose
5 g/L
Yeast extract powder
10 g/L
Calcium chloride
0.008 g/L
Magnesium sulfate
0.008 g/L
Dipotassium phosphate
0.04 g/L
Monopotassium phosphate
0.04 g/L
Sodium bicarbonate
0.4 g/L
Sodium chloride
0.08 g/L
Features

Key features

  • 01

    Lactic acid bacteria counts

    Published for colony counting of lactic acid bacteria in fermented beverages.

  • 02

    Supplement-ready

    Add one vial each of vitamin K1 and hemin chloride solution per 100 mL of sterilized medium.

  • 03

    Simple base preparation

    36 g per litre, boiled and autoclaved at 121 °C for 15 minutes.

  • 04

    Two forms

    Dehydrated powder GF1083F and granulated KL1083F.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.