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Reference: GF1046F / GF618F / KL1046F

Rappaport-Vassiliadis Broth

Selective enrichment broth for Salmonella spp. based on soy peptone, magnesium chloride and malachite green, available as powder or granules.

Key specifications

Product code (dehydrated powder)
GF1046F
Product code (dehydrated powder, alternate listing)
GF618F
Product code (granulated)
KL1046F
Form
Dehydrated powder (GF) and granulated (KL) versions
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Rappaport-Vassiliadis Broth is used for the selective enrichment of Salmonella. It combines soy peptone and phosphate buffer with a high magnesium chloride content (13.58 g/L) and 0.036 g/L malachite green, and has a low final pH of 5.2 ± 0.2.

Suspend 27.1 g in 1 L of water, heat with frequent agitation and boil to dissolve. For the GF1046F and KL1046F listings the broth is divided into test tubes at 10 mL per tube and autoclaved at 115 °C for 15 minutes, then cooled to room temperature. The GF618F listing gives the same dissolution step and the same 115 °C, 15-minute autoclave cycle.

The three catalogue codes share one published composition and a 3-year sealed shelf life. The GF618F listing specifies storage in a dark, dry place at 2-25 °C. The product is not for clinical use.

Specifications

Technical specifications

16 parameters

Identification

Product code (dehydrated powder)
GF1046F
Product code (dehydrated powder, alternate listing)
GF618F
Product code (granulated)
KL1046F
Form
Dehydrated powder (GF) and granulated (KL) versions

Preparation

Quantity per litre of water (distilled, deionized or purified)
27.1 g
Dissolution
Heat with frequent agitation and boil to dissolve completely
Dispensing and sterilisation
Dispense 10 mL per tube (GF1046F / KL1046F); autoclave at 115 °C for 15 minutes; GF618F: dispense and autoclave at 115 °C for 15 minutes
Final pH (25 °C)
5.2 ± 0.2

Storage

Storage
GF1046F / KL1046F: dry place away from light, cap tightened after use. GF618F: dark, dry place at 2-25 °C, bottle cap tightened after use
Shelf life (sealed)
3 years

Composition

Soy Peptone
4.5 g/L
Anhydrous Magnesium Chloride
13.58 g/L
Sodium Chloride
8.0 g/L
Dipotassium Phosphate
0.4 g/L
Potassium Dihydrogen Phosphate
0.6 g/L
Malachite Green
0.036 g/L
Features

Key features

  • 01

    Salmonella enrichment

    Used for selective enrichment of Salmonella spp.

  • 02

    Malachite green selectivity

    Contains malachite green 0.036 g/L and magnesium chloride 13.58 g/L at pH 5.2.

  • 03

    Tube-ready preparation

    Divide into 10 mL tubes and autoclave at 115 °C for 15 minutes.

  • 04

    Powder and granulated

    Dehydrated (GF) and granulated (KL) versions are both listed.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.