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Reference: GF1278F / GF1278EF

SOB (Super Optimal Broth) Medium

Dehydrated SOB broth for E. coli cultivation and for preparing and recovering competent cells after transformation.

Key specifications

Product code
GF1278F (500 g/bottle); GF1278EF (10 kg/bucket)
Form
Dehydrated powder
Preparation
Suspend 28.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
Final pH (25 °C)
7.0 ±0.2
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

SOB (Super Optimal Broth) is used for cultivating E. coli and for preparing and transforming competent cells to improve transformation efficiency, with references to Molecular Cloning: A Laboratory Manual and Hanahan (1983). It is also described as ideal for recovering electrocompetent or chemically competent cells after transformation.

Tryptone and yeast extract supply the nutrients, sodium chloride maintains osmotic balance, magnesium sulfate provides Mg²⁺ to improve membrane permeability and stability, and potassium chloride provides K⁺ that supports enzyme activity. Suspend 28.0 g in 1 L of purified water and autoclave.

Specifications

Technical specifications

11 parameters

General

Product code
GF1278F (500 g/bottle); GF1278EF (10 kg/bucket)
Form
Dehydrated powder
Preparation
Suspend 28.0 g in 1 L of purified water; heat with frequent agitation and boil to dissolve; autoclave at 121 °C for 20 minutes
Final pH (25 °C)
7.0 ±0.2
Reference
Molecular Cloning: A Laboratory Manual; Hanahan, D. (1983) J. Mol. Biol. 166(4), 557-580
Shelf life
3 years

Composition

Tryptone
20 g/L
Yeast Extract
5 g/L
Sodium Chloride
0.5 g/L
Potassium Chloride
0.186 g/L
Magnesium Sulfate
2.4 g/L
Features

Key features

  • 01

    Competent-cell preparation

    Used to prepare and transform competent cells and improve transformation efficiency.

  • 02

    Recovery after transformation

    Described as ideal for recovering electrocompetent or chemically competent cells.

  • 03

    Defined ion supplements

    Mg²⁺ supports membrane permeability and stability; K⁺ supports enzyme activity.

  • 04

    Simple preparation

    28.0 g per litre of purified water, autoclaved at 121 °C for 20 minutes.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.