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Al-Shaker for Scientific and Medical Supplies

Reference: GF961F-GF967F

Synthetic Complete (SC) Medium

Carbon-source-free yeast selective basal medium (YNB plus DO supplement) for cultivating and screening auxotrophic yeast strains.

Key specifications

Form
Dehydrated powder; supplied as SC dropout broths
Preparation
Suspend 4 g of SC broth in 450 mL of distilled water; sterilise by filtration or autoclave at 121 °C for 15 minutes; add 50 mL of sterilised 20% dextrose (or other carbon source) before use
Final pH
5.8 ±0.2
Shelf life
3 years
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Synthetic Complete (SC) medium is used for the selective cultivation and screening of yeast strains with specific amino acid auxotrophies. It is especially suited to experiments that are sensitive to dextrose caramelisation and need high precision.

The medium contains no carbon source, so it can be supplemented with glucose, galactose, raffinose, fructose or sucrose as the experiment requires. It consists of YNB, amino acids and nucleotides: YNB provides growth factors and salts, ammonium sulfate is the nitrogen source, and the DO supplement selectively omits nutrients to create auxotrophic selection or reporter activation. Dextrose is added after sterilisation.

Specifications

Technical specifications

10 parameters

General

Form
Dehydrated powder; supplied as SC dropout broths
Preparation
Suspend 4 g of SC broth in 450 mL of distilled water; sterilise by filtration or autoclave at 121 °C for 15 minutes; add 50 mL of sterilised 20% dextrose (or other carbon source) before use
Final pH
5.8 ±0.2
Shelf life
3 years
Packing
5 L/bottle

Catalog numbers

Single and double dropouts
SC-Leu GF961F; SC-Trp GF962F; SC-Ura GF963F; SC-Ade GF964F; SC-His GF965F; SC-Leu-Trp GF966F
Quadruple dropout
SC-Leu-Trp-His-Ade GF967F

Composition

YNB Powder
6.7 g/L
DO Supplement
1.3 g/L
Agar (optional)
20 g/L
Features

Key features

  • 01

    Flexible carbon source

    Supplement with glucose, galactose, raffinose, fructose or sucrose as needed.

  • 02

    Auxotrophic selection

    DO supplement omits chosen nutrients for auxotrophic selection or reporter gene activation.

  • 03

    Precision work

    Suited to experiments sensitive to dextrose caramelisation.

  • 04

    Seven SC broths

    SC-Leu, -Trp, -Ura, -Ade, -His, -Leu-Trp and -Leu-Trp-His-Ade, in 5 L bottles.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.