Skip to content
Al-Shaker for Scientific and Medical Supplies

Reference: GF946F-GF959F

Synthetic Defined (SD) Premixes

Pre-weighed 24 g SD premix pouches for Saccharomyces cerevisiae and Pichia pastoris, available as broths and agars in seven dropout formats.

Key specifications

Form
Premix bag of 24 g, for 500 mL
Preparation
Suspend 1 bag of SD premix (24 g) in 500 mL of distilled water; sterilise by filtration or autoclave at 121 °C for 15 minutes
Final pH
5.8 ±0.2
Shelf life
3 years
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

Synthetic defined (SD) medium is a fully synthetic yeast medium made of YNB, dextrose, 11 amino acids and nucleotide molecules. Each SD premix lacks one or more specific nutrients, which allows selection of yeast strains able to express the missing component or in which a matching reporter gene is activated.

The premixes are used for cultivating Saccharomyces cerevisiae and Pichia pastoris. YNB provides growth factors and inorganic salts, ammonium sulfate serves as the nitrogen source and dextrose as the fermentable carbon source. Suspend one 24 g bag in 500 mL of distilled water and sterilise.

Specifications

Technical specifications

11 parameters

General

Form
Premix bag of 24 g, for 500 mL
Preparation
Suspend 1 bag of SD premix (24 g) in 500 mL of distilled water; sterilise by filtration or autoclave at 121 °C for 15 minutes
Final pH
5.8 ±0.2
Shelf life
3 years
Packing
0.5 L/pouch, 5 pouches/box

Catalog numbers

SD broths
SD-Leu GF946F; SD-Trp GF947F; SD-Ura GF948F; SD-Ade GF949F; SD-His GF950F; SD-Leu-Trp GF951F; SD-Leu-Trp-His-Ade GF952F
SD agars
SD-Leu GF953F; SD-Trp GF954F; SD-Ura GF955F; SD-Ade GF956F; SD-His GF957F; SD-Leu-Trp GF958F; SD-Leu-Trp-His-Ade GF959F

Composition

YNB Powder
6.7 g/L
DO Supplement
1.3 g/L
Dextrose (D-Glucose)
20 g/L
Agar (optional)
20 g/L
Features

Key features

  • 01

    Pre-weighed pouches

    One 24 g bag makes 500 mL, with no need to blend components.

  • 02

    Selective dropouts

    SD-Leu, -Trp, -Ura, -Ade, -His, -Leu-Trp and -Leu-Trp-His-Ade.

  • 03

    Broth and agar versions

    Seven broths and seven agars.

  • 04

    Yeast species

    For Saccharomyces cerevisiae and Pichia pastoris.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.