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Reference: GF1029F / KL1029F
Tetrathionate Broth Base
Base medium for the selective enrichment of Salmonella, completed with brilliant green and iodine solution or with neomycin for MKTTn broth.
Key specifications
- Product code (dehydrated powder)
- GF1029F
- Product code (granulated)
- KL1029F
- Form
- Dehydrated powder (GF) and granulated (KL) versions
- Quantity per litre of distilled or deionized water
- 93.6 g
- Configured to your application by our specialists
- Installed and commissioned on site
- Training and after-sales support across Jordan, the GCC and MENA
Tetrathionate Broth Base
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Product overview
Tetrathionate Broth Base is the basal medium for selective enrichment of Salmonella. It contains peptone, beef extract, sodium chloride, calcium carbonate, sodium thiosulfate and ox bile salts, and is completed at the time of use with separately supplied reagents.
Suspend 93.6 g in 1 L of distilled or deionized water, heat with frequent agitation and boil to dissolve, without autoclaving. After cooling to 30 °C, add two bottles of sodium tetrathionate brilliant green enrichment solution (one brilliant green and one iodine solution) per 100 mL of base, or two bottles of neomycin to obtain MKTTn broth. Medium containing brilliant green and iodine should be used on the same day and must not be heated again.
The final pH is 7.6 ± 0.2. The source lists powder (GF1029F) and granulated (KL1029F) versions with identical composition.
Specifications
Technical specifications
16 parameters
Identification
- Product code (dehydrated powder)
- GF1029F
- Product code (granulated)
- KL1029F
- Form
- Dehydrated powder (GF) and granulated (KL) versions
Preparation
- Quantity per litre of distilled or deionized water
- 93.6 g
- Dissolution
- Heat with frequent agitation and boil to dissolve completely; do not autoclave
- Supplementation
- Cool to 30 °C; add 2 bottles of sodium tetrathionate brilliant green enrichment solution (one brilliant green, one iodine solution) per 100 mL base, or 2 bottles of neomycin to make MKTTn broth
- Use of finished medium
- Medium containing brilliant green and iodine solution must be used the same day and not reheated
- Final pH (25 °C)
- 7.6 ± 0.2
Storage
- Storage
- Store in a dry place away from light; tighten the cap immediately after use
- Shelf life (sealed)
- 3 years
Composition
- Peptone
- 9 g/L
- Beef Extract
- 4.5 g/L
- Sodium Chloride
- 2.7 g/L
- Calcium Carbonate
- 40.5 g/L
- Anhydrous Sodium Thiosulfate
- 31.9 g/L
- Ox Bile Salts
- 5 g/L
Features
Key features
- 01
Salmonella enrichment
Intended for the selective enrichment of Salmonella.
- 02
Two supplement routes
Add brilliant green plus iodine solution, or neomycin to make MKTTn broth.
- 03
No autoclaving
The base is boiled to dissolve and is not autoclaved.
- 04
Same-day use
Medium with brilliant green and iodine should be used on the day it is prepared and not reheated.
- 05
Safe handling
Wear a mask when weighing; autoclave used material at 121 °C for 30 minutes before disposal.
From order to operation
We stay with the instrument after it arrives
Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.
- 01
Consult
- 02
Plan
- 03
Supply
- 04
Install
- 05
Train
- 06
Support
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