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Reference: GF1012F / KL1012F
Triple Sugar Iron Agar (TSI), GF1012F / KL1012F
Slant agar with lactose, sucrose and glucose for the biochemical identification of Salmonella and other Gram-negative Enterobacteriaceae; ISO 6579 is listed.
Key specifications
- Product code (dehydrated powder)
- GF1012F
- Product code (granulated)
- KL1012F
- Form
- Dehydrated powder (GF) and granulated (KL) versions
- Standards (ACC field)
- ISO 6579 (GF1012F)
- Configured to your application by our specialists
- Installed and commissioned on site
- Training and after-sales support across Jordan, the GCC and MENA
Triple Sugar Iron Agar (TSI), GF1012F / KL1012F
Request a quoteOverview
Product overview
Triple Sugar Iron Agar is used for biochemical identification of Salmonella and other Enterobacteriaceae. It combines lactose, sucrose and glucose with an iron salt and sodium thiosulfate and phenol red as the indicator, and is poured as slants with deep butts in test tubes. The GF1012F listing references ISO 6579.
The powder (GF1012F) and granulated (KL1012F) versions have somewhat different published formulas and are therefore described separately in the specifications. GF1012F uses 64.6 g/L, autoclaved at 121 °C for 15 minutes, with beef extract 3.0, yeast extract 3.0 and peptone 20.0 g/L. KL1012F uses 63.4 g/L, autoclaved at 115 °C for 15 minutes, with peptone 20.0 and beef extract 5.0 g/L and ammonium ferrous sulfate as the iron source. Both end at pH 7.4 ± 0.2.
After autoclaving, tubes are cooled in a slanted position. Sealed shelf life is 3 years. Not for clinical use.
Specifications
Technical specifications
34 parameters
Identification
- Product code (dehydrated powder)
- GF1012F
- Product code (granulated)
- KL1012F
- Form
- Dehydrated powder (GF) and granulated (KL) versions
- Standards (ACC field)
- ISO 6579 (GF1012F)
Preparation
- Quantity per litre of purified water (GF1012F)
- 64.6 g
- Quantity per litre of purified water (KL1012F)
- 63.4 g
- Dissolution
- Heat with frequent agitation and boil to dissolve completely; distribute into test tubes
- Sterilisation (GF1012F)
- Autoclave at 121 °C for 15 minutes
- Sterilisation (KL1012F)
- Autoclave at 115 °C for 15 minutes
- Setting
- Cool tubes in a slanted position to form slants with deep butts
- Final pH (25 °C), both versions
- 7.4 ± 0.2
Storage
- Storage
- Store in a dark, dry place at 2-25 °C; tighten the bottle cap immediately after use
- Shelf life (sealed)
- 3 years
Composition
- Beef extract (GF1012F)
- 3.0 g/L
- Yeast extract (GF1012F)
- 3.0 g/L
- Peptone (GF1012F)
- 20.0 g/L
- Sodium chloride (GF1012F)
- 5.0 g/L
- Lactose (GF1012F)
- 10.0 g/L
- Sucrose (GF1012F)
- 10.0 g/L
- Glucose (GF1012F)
- 1.0 g/L
- Iron(III) citrate (GF1012F)
- 0.3 g/L
- Sodium thiosulfate (GF1012F)
- 0.3 g/L
- Phenol red (GF1012F)
- 0.024 g/L
- Agar (GF1012F)
- 12.0 g/L
- Peptone (KL1012F)
- 20.0 g/L
- Beef extract (KL1012F)
- 5.0 g/L
- Lactose (KL1012F)
- 10 g/L
- Sucrose (KL1012F)
- 10 g/L
- Dextrose (KL1012F)
- 1 g/L
- Sodium chloride (KL1012F)
- 5 g/L
- Ammonium ferrous sulfate (KL1012F)
- 0.2 g/L
- Sodium thiosulfate (KL1012F)
- 0.2 g/L
- Agar (KL1012F)
- 12 g/L
- Phenol red (KL1012F)
- 0.025 g/L
Features
Key features
- 01
Biochemical identification
Used for biochemical identification of Salmonella and other Gram-negative Enterobacteriaceae.
- 02
Three sugars
Lactose 10, sucrose 10 and glucose or dextrose 1 g/L.
- 03
Slant with deep butt
Distributed into test tubes and cooled in a slanted position.
- 04
ISO reference
The GF1012F listing cites ISO 6579.
- 05
Version-specific preparation
GF1012F: 64.6 g/L at 121 °C; KL1012F: 63.4 g/L at 115 °C, each for 15 minutes.
From order to operation
We stay with the instrument after it arrives
Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.
- 01
Consult
- 02
Plan
- 03
Supply
- 04
Install
- 05
Train
- 06
Support
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