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Reference: GF1002F / KL1002F
Violet Red Bile Lactose (VRBL) Agar
Violet red bile lactose agar for cultivating and counting Enterobacteriaceae, referenced to ISO 4832, prepared by boiling for two minutes without autoclaving.
Key specifications
- Product code (dehydrated powder)
- GF1002F
- Product code (granulated)
- KL1002F
- Form
- Dehydrated powder and granulated forms
- Standard reference (ACC)
- ISO 4832
- Configured to your application by our specialists
- Installed and commissioned on site
- Training and after-sales support across Jordan, the GCC and MENA
Violet Red Bile Lactose (VRBL) Agar
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Product overview
Violet Red Bile Lactose (VRBL) Agar is used for the cultivation and enumeration of Enterobacteriaceae and carries an ISO 4832 reference in the manufacturer's data. The formulation combines an enzymatic digest of animal tissues, yeast extract, lactose, bile salts, neutral red and crystal violet in an agar base (final pH 7.4±0.2).
Suspend 41.5 g in 1 L of purified water and boil with frequent agitation for two minutes; autoclaving is not needed. Cool the medium immediately in a 44-47 °C water bath and use it within 4 hours of preparation. Dehydrated powder (GF1002F) and granulated (KL1002F) forms are stored at 2-25 °C with the bottle cap tightened after use and have a 3-year sealed shelf life.
Specifications
Technical specifications
16 parameters
Product
- Product code (dehydrated powder)
- GF1002F
- Product code (granulated)
- KL1002F
- Form
- Dehydrated powder and granulated forms
- Standard reference (ACC)
- ISO 4832
Preparation
- Preparation
- Suspend 41.5 g in 1 L of purified water; heat with frequent agitation and boil for two minutes to dissolve; no autoclaving needed; cool immediately in a 44-47 °C water bath and use within 4 h of preparation.
- Final pH (25 °C)
- 7.4±0.2
Storage
- Storage
- Store in a dark and dry place at 2-25 °C; tighten the bottle cap immediately after use.
- Shelf life (sealed)
- 3 years
Composition
- Enzymatic digest of animal tissues
- 7.0 g/L
- Yeast extract
- 3.0 g/L
- Lactose
- 10.0 g/L
- Sodium chloride
- 5.0 g/L
- Bile salts
- 1.5 g/L
- Neutral red
- 0.03 g/L
- Crystal violet
- 0.002 g/L
- Agar
- 15.0 g/L
Features
Key features
- 01
Enterobacteriaceae enumeration
Published for cultivation and enumeration of Enterobacteriaceae.
- 02
ISO 4832 reference
The product data lists ISO 4832 as its standard reference.
- 03
No autoclaving
Boil for two minutes, then cool in a 44-47 °C water bath.
- 04
Use within 4 hours
Prepared medium should be used within 4 hours of preparation.
From order to operation
We stay with the instrument after it arrives
Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.
- 01
Consult
- 02
Plan
- 03
Supply
- 04
Install
- 05
Train
- 06
Support
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