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Reference: RB0201

10X ViBuffer A, 10X ViBuffer S and 50 mM MgCl2

Ready-made PCR reaction buffers (ViBuffer A for general PCR, ViBuffer S for long PCR) and 50 mM MgCl2 solution, each in 5 x 1 ml packs.

Key specifications

Cat. No. RB0201
10X ViBuffer A - 5x1ml
Cat. No. RB0203
10X ViBuffer S - 5x1ml
Cat. No. RB0204
50mM MgCl2 - 5x1ml
10X ViBuffer A
500 mM KCl, 100 mM Tris-HCl (pH 9.1 at 20 C), 0.1% Triton X-100
  • Configured to your application by our specialists
  • Installed and commissioned on site
  • Training and after-sales support across Jordan, the GCC and MENA

Overview

Product overview

These standalone PCR reagents let you adjust or rebuild reactions. 10X ViBuffer A (500 mM KCl, 100 mM Tris-HCl pH 9.1, 0.1% Triton X-100) is a general buffer for various PCR reactions, optimised for 0.1-0.2 mM of each dNTP.

10X ViBuffer S (160 mM (NH4)2SO4, 500 mM Tris-HCl pH 9.2, 17.5 mM MgCl2, 0.1% Triton X-100) is meant for long PCR and is optimised for 0.35 mM of each dNTP. A separate 50 mM MgCl2 solution is offered for fine-tuning magnesium concentration.

Specifications

Technical specifications

6 parameters

Ordering information

Cat. No. RB0201
10X ViBuffer A - 5x1ml
Cat. No. RB0203
10X ViBuffer S - 5x1ml
Cat. No. RB0204
50mM MgCl2 - 5x1ml

Composition

10X ViBuffer A
500 mM KCl, 100 mM Tris-HCl (pH 9.1 at 20 C), 0.1% Triton X-100
10X ViBuffer S
160 mM (NH4)2SO4, 500 mM Tris-HCl (pH 9.2 at 22 C), 17.5 mM MgCl2, 0.1% Triton X-100
50 mM MgCl2
50 mM MgCl2.6H2O
Features

Key features

  • 01

    10X ViBuffer A

    General PCR buffer, 0.1-0.2 mM dNTP.

  • 02

    10X ViBuffer S

    Long PCR buffer, 0.35 mM dNTP.

  • 03

    50 mM MgCl2

    For optimising magnesium concentration.

From order to operation

We stay with the instrument after it arrives

Al-Shaker specifies the configuration, documents the system, and supports it across Jordan, the GCC, and MENA.

  1. 01

    Consult

  2. 02

    Plan

  3. 03

    Supply

  4. 04

    Install

  5. 05

    Train

  6. 06

    Support

Talk to the technical team

Tell us the application. We will specify the system.

Share the workflow, capacity, and compliance constraints. We respond with a recommended configuration, not a generic catalogue list.