pTG19-T PCR cloning vector

Vivantis pTG19-T (TA010): linearized 3′-dT vector for 3′-dA PCR products — >80% recombinants, lacZ screening, BamHI insert release, 20 applications.

Key specifications

Ends: 3′-dT overhangs
Recombinants: >80%
Marker: lacZ blue/white
Enzyme: BamHI

Configured to your application by our specialists
Installed and commissioned on site
Training and after-sales support across Jordan, the GCC and MENA

pTG19-T PCR cloning vector

Overview

Product overview

The pTG19-T vector is linearized with 3′-dT overhangs so PCR products with 3′-dA ends clone without recircularization of empty vector. Vivantis publishes more than 80% of recombinant clones containing the target DNA, lacZ for blue/white selection, M13 primer sites for PCR screening and sequencing, and BamHI sites to release the insert. Catalogue TA010 is 20 applications. Quality notes on the product page include more than 80% white colonies with the control insert and confirmation of 3′-dT overhangs by sequencing.

Al-Shaker specifies TA010 for TA cloning of Taq products (including PLMM01 amplicons), then supplies the vector across Jordan, the GCC, and MENA.

Specifications

Technical specifications

5 parameters

Ends: 3′-dT overhangs
Recombinants: >80%
Marker: lacZ blue/white
Enzyme: BamHI
TA010: 20 applications

Features

Key features

01
3′-dT, >80% recombinants
Linearized T-overhangs block empty recircularization; Vivantis publishes >80% target inserts.
02
lacZ and BamHI
Blue/white on lacZ; BamHI releases the insert; M13 sites for colony PCR and sequencing.
03
TA010, 20 applications
One published pack size on the Vivantis cloning page.